Mass spectrometry-based proteomics analysis of whole cell and secreted extracellular vesicles of four diffuse large B-cell lymphoma cell lines / Henrique Silva Caio Simões Baeta ; orient. Rune Matthiesen, Ana Sofia Carvalho
Main Author Baeta, Henrique Silva Caio Simões Secondary Author Matthiesen, RuneCarvalho, Ana Sofia Publication Lisboa : NOVA Medical School, 2021 Description 56 p : il. Abstract DISCUSSION: Here we implemented an experimental and computational workflow to study the protein expression profiles of small extracellular vesicles from in vitro cultured DLBCL cell lines. Our main question was whether the proteomes of small extracellular vesicles could be used to segregate the prognostic cell of origin subtypes of DLBCL. We selected 4 DLBCL cell line models, the DB and HT cells representing germinal center b cell like (GCB) and RIVA and OCI-ly3 cells as activated b cell like (ABC) DLBCLs. We cultured cells considering suppliers instructions to make sure our results are comparable to others using the same models. We tried to truthfully report our experimental conditions and sEVs purification protocol to prevent possible misinterpretation of data. To our knowledge this is the first high accuracy, global, quantitative proteomics study to comprehensively compare the proteomes of sEVs derived from DLBCL cells. We considered the expression of other biomolecules in sEVs. However, proteins are main effectors of encoding genes and among the most frequently therapeutically targeted biomolecules. We decided to take advantage of recent developments in mass spectrometry instruments147 and data analysis strategies127 to investigate DLBCL sEVs proteomes. Topical name B-cell Lymphoma
Cell Lines
Proteomics
Academic Dissertation Index terms Universidade NOVA de Lisboa
NOVA Medical School
Dissertação de Mestrado
Investigação Biomédica
2021 CDU 616 Online Resources Click here to access the eletronic resource http://hdl.handle.net/10362/118632
| Item type | Current location | Call number | url | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Documento Eletrónico | Biblioteca NMS|FCM online | RUN | http://hdl.handle.net/10362/118632 | Available | 20210322 |
DISCUSSION: Here we implemented an experimental and computational workflow to study the protein expression profiles of small extracellular vesicles from in vitro cultured DLBCL cell lines. Our main question was whether the proteomes of small extracellular vesicles could be used to segregate the prognostic cell of origin subtypes of DLBCL. We selected 4 DLBCL cell line models, the DB and HT cells representing germinal center b cell like (GCB) and RIVA and OCI-ly3 cells as activated b cell like (ABC) DLBCLs. We cultured cells considering suppliers instructions to make sure our results are comparable to others using the same models. We tried to truthfully report our experimental conditions and sEVs purification protocol to prevent possible misinterpretation of data. To our knowledge this is the first high accuracy, global, quantitative proteomics study to comprehensively compare the proteomes of sEVs derived from DLBCL cells. We considered the expression of other biomolecules in sEVs. However, proteins are main effectors of encoding genes and among the most frequently therapeutically targeted biomolecules. We decided to take advantage of recent developments in mass spectrometry instruments147 and data analysis strategies127 to investigate DLBCL sEVs proteomes.
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