The role of extracellular vesiclesin multiple myeloma / Bruna Velosa Ferreira ; orient. Cristina Maria Godinho Pires João, Bruno Costa-Silva
Main Author Ferreira, Bruna Velosa Secondary Author João, Cristina Maria Godinho PiresCosta-Silva, Bruno Language Inglês. Country Portugal. Publication Lisboa : NOVA Medical School, Universidade NOVA de Lisboa, 2022 Dissertation Note or Thesis: Tese de Doutoramento
Medicina, Investigação Clínica
2022
Faculdade de Ciências Médicas, Universidade NOVA de Lisboa
Abstract Abstract: Multiple myeloma (MM) is a hematological malignancy of clonal antibody-secreting plasma-cells(PC). MM diagnosis and risk stratification rely on tumoral assessment via bone marrow (BM) biopsy, which is an invasive procedure prone to sample bias. Liquid biopsies, such as extracellular vesicles (EV) in peripheral blood(PB), hold promise as new minimallyinvasive tools. Real-world studies analyzing patient-derived EV proteome are rare. Here, we characterizePB and BMEV protein content from acohort of 102monoclonal gammopathiespatients routinely followed in theclinic. A total of 223 PB and 111BM samples were included.We investigated whether EV protein and particle concentrationcould predict MM patient prognosisandfoundthat high EVprotein/particle (EVc> 0.6 μg/108particles) levelis related to poorer survival and immune dysfunction.These results were supportedat protein level by mass spectrometry.We report a set of PB EV-proteins (PDIA3, C4BPA, BTN1A1, APRIL, PSMB8 and PDE8B) with new biomarker potential for myeloma patientoutcomes. High proteomic expression similarity between PB and BM matched pairs were found, suggestingthe use of circulating EV as a personalized counterpart of BM EV proteome. Overall, we found that EV protein content couldberelated to patient outcomes, suchassurvival,immune dysfunction,andpossiblyspecific treatment response. Topical name Multiple Myeloma
Extracellular Vesicles
Academic Dissertation Index terms Medicina
NOVA Medical School
2022 Online Resources Click here to access the eletronic resource http://hdl.handle.net/10362/136069
| Item type | Current location | Call number | url | Status | Date due | Barcode |
|---|---|---|---|---|---|---|
| Documento Eletrónico | Biblioteca NMS|FCM online | RUN | http://hdl.handle.net/10362/136069 | Available | 20220092 |
Tese de Doutoramento Medicina, Investigação Clínica 2022 Faculdade de Ciências Médicas, Universidade NOVA de Lisboa
Abstract: Multiple myeloma (MM) is a hematological malignancy of clonal antibody-secreting plasma-cells(PC). MM diagnosis and risk stratification rely on tumoral assessment via bone marrow (BM) biopsy, which is an invasive procedure prone to sample bias. Liquid biopsies, such as extracellular vesicles (EV) in peripheral blood(PB), hold promise as new minimallyinvasive tools. Real-world studies analyzing patient-derived EV proteome are rare. Here, we characterizePB and BMEV protein content from acohort of 102monoclonal gammopathiespatients routinely followed in theclinic. A total of 223 PB and 111BM samples were included.We investigated whether EV protein and particle concentrationcould predict MM patient prognosisandfoundthat high EVprotein/particle (EVc> 0.6 μg/108particles) levelis related to poorer survival and immune dysfunction.These results were supportedat protein level by mass spectrometry.We report a set of PB EV-proteins (PDIA3, C4BPA, BTN1A1, APRIL, PSMB8 and PDE8B) with new biomarker potential for myeloma patientoutcomes. High proteomic expression similarity between PB and BM matched pairs were found, suggestingthe use of circulating EV as a personalized counterpart of BM EV proteome. Overall, we found that EV protein content couldberelated to patient outcomes, suchassurvival,immune dysfunction,andpossiblyspecific treatment response.
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