000			nam a22 4500
001			15816
100			_a20230126 pory50
200			_aThe influence of anthocyanin treatment on the mesenteric adipose tissue expression of enampt in animal models of obesity _fMaria Margarida Nobre Projecto _gorient. Marta P. Silvestre, Ana Faria
210			_aLisboa _cNOVA Medical School, Universidade NOVA de Lisboa _d2022
215			_a43 p.
328			_bDissertação de Mestrado _cNutrição Humana e Metabolismo _d2022 _eFaculdade de Ciências Médicas, Universidade NOVA de Lisboa
330			_aIntroduction: Nicotinamide Phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme for NAD biosynthesis. Its extracellular form (eNAMPT), mainly secreted by visceral fat, has been shown to have bimodal, concentration- and structure-functional-dependent effects in important metabolic pathways and has been connected to a wide variety of diseases. Data suggests that as serum eNAMPT concentration rises to pathophysiological levels, as in obesity and type 2 diabetes (T2D), eNAMPT adopts a monomeric form capable of proinflammatory NAD-independent effects. Strategies to block the actions of the eNAMPT monomer could represent promising therapeutic approaches for obesity-related metabolic disorders. Consumption of anthocyanin rich foods appears to prevent or treat obesity-related consequences, such as T2D, inflammation and oxidative stress, but the mechanism behind this is unknown. Moreover, anthocyanins have been shown to inhibit the secretion of eNAMPT in animal models. With this study, we aim to understand if treating a rat model of obesity with anthocyanins could abrogate the impact of a high fat diet in the expression of monomeric eNAMPT. Methods: Mesenteric adipose tissue (mAT) was obtained from four groups of male Wistar rats, treated with different diets: (C) standard diet; (BE) standard diet + blackberry extract; (HFD) high fat diet; (HFDBE) high-fat diet + blackberry extract. eNAMPT monomer’s protein expression was measured by Western Blotting, after protein extraction and quantification from mAT, to access the differences between the animals fed a standard diet and those of increased metabolic risk – HFD, with and without treatment with anthocyanins. Results: The mAT from HFD rats displayed a higher expression of eNAMPT monomer, compared to C rats (138,6% ± 11,4% HFBE vs 100% C, p=0,01). The anthocyanin’s treatment influence on mAT eNAMPT monomer expression was also assessed. mAT eNAMPT monomer expression was significantly decreased in the HFDBE group compared to HFD (-54,1 ± 15,3 % [-89.4, -18.8], p<0,01). Conclusions: Anthocyanin consumption might be an interesting dietary approach to abrogate the impact of a high fat diet on the rise of monomeric eNAMPT in mesenteric adipose tissue. Ultimately, our results suggest that long-term anthocyanin treatment/supplementation might be effective for sustaining lower levels of monomeric eNAMPT in the context of diet-induced obesity, potentially preventing or delaying the consequent metabolic impairments such as the alarming epidemic of T2D.
606			_aAdipose Tissue
606			_aAnthocyanins
606			_aeNAMPT
606			_aNicotinamide Phosphoribosyltransferase
606			_aObesity
606			_aDiabetes Mellitus, Type 2
700			_aProjecto _bMaria Margarida Nobre
702			_4727 _922200 _aSilvestre _bMarta P.
702			_4727 _921458 _aFaria _bAna
801			_aPT _bNMS _gRPC
856			_uhttp://hdl.handle.net/10362/148130
090			_a15816
942			_cDLEC _n0